![]() ![]() We further demonstrated that exosomal THBS1 mediates these stiffness-induced effects by engaging matrix metalloproteinase and focal adhesion kinase. ![]() Knockdown studies confirmed that the pro-invasive effects of stiffness-tuned exosomes are fuelled by exosomal THBS1. Quantitative proteomics of exosomes combined with genomic analysis of human breast tumor tissues (TCGA database) identified thrombospondin-1 (THBS1) as a prospective regulator of stiffness-dependent cancer invasion. Proteomic analysis of exosomal lysates followed by overrepresentation analysis and interactome studies revealed enrichment of cell adhesion and cell migration proteins in exosomes from stiff ECM cultures compared to that of soft ones. Reciprocally, exogenous addition of ECM stiffness-tuned exosomes orchestrated a series of changes in cell morphology, adhesion, protrusion dynamics resulting in fostered cell motility and invasion. Interestingly, blocking exosome synthesis and secretion by GW4869 abrogated stiffness regulated motility and contractility in breast cancer cells. Using stiffness tuneable hydrogel ECM scaffolds, we show that stiff ECMs promote exosome secretion in a YAP/TAZ pathway-dependent manner. Here we demonstrate the role of exosomes in ECM stiffness triggered breast cancer invasiveness. The mechanisms by which extracellular matrix (ECM) stiffening drives breast cancer invasion is an area of active research. The crocodile probe is the common terminal.Breast cancer progression features ECM stiffening due to excess deposition and crosslinking of collagen, which dramatically influence tumor behaviour and fate. Yellow ring Probe indicates channel 1 blue ring probe indicates channel 2. Connections: Probes can be connected to each socket.Frequency (Digital): From 0.2 kHz to 25 kHz.Input Voltage: Probe 1X: ±5 V Probe 10X: ±50 V.Automatic selection steps of Voltage, current, frequency. Test resistance: Current level: low 47 k?, medium 2,6 k?, high 385 ?. ![]() While using TEST feature, a comparison can be conducted without giving energy to the card while using OSCILLOSCOPE feature, energy is given to card and signals are controlled. It can also be used as a component tester for IC, transistor, FET, IGBT, resistor, capacitor, indicator.FADOS7F1 is a double-channel VI tester. The Fados 7F1 which stands for Fault Detector and Oscilloscope 7 Functions in 1.įADOS 7F1 can be used for determining faults of all types of PCBs present in Electronic devices used in medical, textile industries, Electronic Appliances and other large automated devices used in the Automobile Industry as well. They are basically computer based VI testers. FADOS multifunction circuit board testers have been specially developed by ProT Ar-Ge to determine and troubleshoot faults at all type electronic circuit boards. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
January 2023
Categories |